Enumerix - Partitioning Technology

30+ million partitions

Single molecule PCR

Clear emulsion chemistry

Universal Multiplex™

Comboplex™

30+ million partitions per sample are generated using a standard centrifuge, generating >1500X more partitions than conventional dPCR

Advantages:

Simple design eliminates the need for complex microfluidics, so this process can be performed in most molecular biology labs.
Spin column format enables industry-leading PCR volume of 50uL, suitable for diluted samples such as cell free DNA (cfDNA) in search for rare molecules.
High centrifugal force processes the entire sample with no dead volume, adding to the sensitivity of UltraPCR for rare molecule dection

Efficient partitioning without microfluidics with no dead volume

Centrifugation-based partitioning using a standard benchtop centrifuge
30+ million partitions per sample directly placed into PCR tubes, 1000X+ more partitions than conventional dPCR
Industry-leading 50 uL PCR reactions with no dead volume

Counting DNA the way it should be - 1 molecule at a time.

Singlet partitions in UltraPCR

Mixed molecule partitions in dPCR

30+million partitions enables >1 million molecules, each in its own “singlet” partition
Count with simplicity and confidence - 1 positive singlet partition equals 1 DNA molecule
Eliminate the need of Poisson statistics arising from limited partitions in legacy dPCR systems
True single molecule counting over an unmatched 6-log dynamic range, achieving the true concept of digital PCR

Clarity in 3D provides direct peek of singlet partitions directly from PCR tubes.

Ultra-dPCR

Other dPCR

Proprietary chemistry enables formation of thermally stable and optically clear partitions for rapid, high-resolution 3D scanning in the same tube
Positive partitions are counted directly from within the PCR tube, providing a streamlined, single-tube workflow and eliminating risks of cross-sample contaminations
UltraPCR platform uses lightsheet imaging to provide high resolution 3D microscopy directly in PCR tubes with clear partitions
UltraClear 3D can scan 34 million partitions in under a minute per channel

Bringing multiplexing for everyday use.

Novel multiplexing alternative using a standard pair of PCR primers without the need of expensive hydrolysis probes
Rapid, low cost, multiplexed panel design (up to 4 with standard product offering)
Comparable counting performance as hydrolysis probes

Unprecedented level of multiplexing unlocked in UltraPCR.

A Fundamentally Different Approach