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Unlocking clean PCR, and easy data analysis
30+ million partition capacity spreads DNA targets into single molecule partitions.
Plot displaying the % of partitions encompassing more than 1 molecule in legacy dPCR systems (20k partitions, black) vs UltraPCR (30+ million partitions, blue). In UltraPCR, even at 1,000,000 molecule input, ~99.95% of partitions are either empty or with single target molecules, which enables a paradigm shift of massively parallel single molecule PCR. Schematic of UltraPCR DNA occupancy mimics the actual proportion of partitions with 1 million molecules in 30+ million partitions (2.9% occupancy). On the other hand, in legacy dPCR systems, even with 50,000 molecule input, more than 70% of the partitions are occupied with two or more target molecules (250% occupancy), as shown in the schematic.
Advantages:
Every molecule is amplified without partition-specific competition - a common issue in multiplex PCR - making multiplex assay development more straightforward.
Every fluorescent partition signifies 1 molecule, enabling true digital counting with single molecule precision without any Poisson correction.
Signal from single molecule partition is clean without the ambiguities of mixed molecule partitions observed in conventional dPCR.
UltraPartitioning™
Efficient partitioning without microfluidics with no dead volume
Centrifugation-based partitioning using a standard benchtop centrifuge
30+ million partitions per sample directly placed into PCR tubes, 1000X+ more partitions than conventional dPCR
Industry-leading 50 uL PCR reactions with no dead volume
Singlet Counting™
Counting DNA the way it should be - 1 molecule at a time.
Singlet partitions in UltraPCR
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Mixed molecule partitions in dPCR
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30+million partitions enables >1 million molecules, each in its own “singlet” partition
Count with simplicity and confidence - 1 positive singlet partition equals 1 DNA molecule
Eliminate the need of Poisson statistics arising from limited partitions in legacy dPCR systems
True single molecule counting over an unmatched 6-log dynamic range, achieving the true concept of digital PCR
UltraClear 3D™
Clarity in 3D provides direct peek of singlet partitions directly from PCR tubes.
Ultra-dPCR
Other dPCR
Proprietary chemistry enables formation of thermally stable and optically clear partitions for rapid, high-resolution 3D scanning in the same tube
Positive partitions are counted directly from within the PCR tube, providing a streamlined, single-tube workflow and eliminating risks of cross-sample contaminations
UltraPCR platform uses lightsheet imaging to provide high resolution 3D microscopy directly in PCR tubes with clear partitions
UltraClear 3D can scan 34 million partitions in under a minute per channel
Universal Multiplex™
Bringing multiplexing for everyday use.
Novel multiplexing alternative using a standard pair of PCR primers without the need of expensive hydrolysis probes
Rapid, low cost, multiplexed panel design (up to 4 with standard product offering)
Comparable counting performance as hydrolysis probes
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