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Comboplex™ pushes multiplexing to new heights
Optical signatures collected by UltraPCR Imager enables us to expand the number of biomarkers surveyed per reaction. Using a technique called Comboplex, a target can be labeled with 1 or more fluorescent dyes to create unique optical signatures for biomarker differentiation.
Figure 1. Example of how targets can be uniquely labeled using 2 fluorophores per target. Since UltraPCR operates in a single molecule realm, signal from individual partitions is distinct to the target assigned without ambiguity.
Figure 2. UltraPCR comboplex targets can be labeled uniquely either using 1, 2, or 3 fluorophores, imaged in different channels, and decoded using our 3D imaging analysis algorithm.
Figure 3. Example data from an UltraPCR 22-plex Comboplex assay using 8 fluorophores on a 4-color imager, where each point is a positive DNA molecule. Left, middle, right panel shows simultaneous detection of 4, 8, and 22 pathogens spiked into a single reaction. Despite being a high parameter dataset, UltraPCR's ability to work in a single molecule realm leads to clean target clusters - as visualized in the above UMAP - allowing automatic gating, with minimal human intervention, for rapid data analysis.
UltraPartitioning™
Efficient partitioning without microfluidics with no dead volume
Centrifugation-based partitioning using a standard benchtop centrifuge
30+ million partitions per sample directly placed into PCR tubes, 1000X+ more partitions than conventional dPCR
Industry-leading 50 uL PCR reactions with no dead volume
Singlet Counting™
Counting DNA the way it should be - 1 molecule at a time.
Singlet partitions in UltraPCR
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Mixed molecule partitions in dPCR
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30+million partitions enables >1 million molecules, each in its own “singlet” partition
Count with simplicity and confidence - 1 positive singlet partition equals 1 DNA molecule
Eliminate the need of Poisson statistics arising from limited partitions in legacy dPCR systems
True single molecule counting over an unmatched 6-log dynamic range, achieving the true concept of digital PCR
UltraClear 3D™
Clarity in 3D provides direct peek of singlet partitions directly from PCR tubes.
Ultra-dPCR
Other dPCR
Proprietary chemistry enables formation of thermally stable and optically clear partitions for rapid, high-resolution 3D scanning in the same tube
Positive partitions are counted directly from within the PCR tube, providing a streamlined, single-tube workflow and eliminating risks of cross-sample contaminations
UltraPCR platform uses lightsheet imaging to provide high resolution 3D microscopy directly in PCR tubes with clear partitions
UltraClear 3D can scan 34 million partitions in under a minute per channel
Universal Multiplex™
Bringing multiplexing for everyday use.
Novel multiplexing alternative using a standard pair of PCR primers without the need of expensive hydrolysis probes
Rapid, low cost, multiplexed panel design (up to 4 with standard product offering)
Comparable counting performance as hydrolysis probes
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